eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
1
14
10.22092/ijfs.2018.118930.
117465
مقاله پژوهشی
Fish assemblages and habitat ecology of River Pinder in central Himalaya, India
N K Agarwal
agarwalnareshk3@rediffmail.com
1
U S Rawat
drudairawat@gmail.com
2
Gurunam Singh
fishguru88@yahoo.com
3
H N B Garhwal University India
Sri Dev Sumen uttrakhand University, India
H N B Garhwal University India
Snow-fed river Pinder -a tributary of river Alaknanda in central Himalaya was explored for fish assemblages and habitat specificity. Altogether 27 fish species were reported from three orders, four families and nine genera. Cypriniformes order was dominating followed by Siluriformes and Salmoniformes. Shannon-Weiner diversity index (3.09 to 4.10) and Simpson index of diversity (0.81 to 0.92) of four sites specified strong relationship with species richness. The distribution of fish species showed interesting patterns, 33% species were common to all four sampling sites while 14.80% were restricted to single site and remaining species were randomly distributed among two or three sampling sites. Habitat variability in river significantly influenced the species assemblage structure. About 7.40% species were found common to all habitats while 3.70% species were restricted to only single habitat type. Remaining 88.90% species were dwelling between two to three habitat types. Deep pools recorded maximum species richness followed by shallow pool, while least species richness was recorded from cascade habitat. The conservation status of fish fauna of river was ascertained by CAMP (1998) assessment. Out of 27 species, status of 8 species was not assessed due to data deficient, 7 species were categorised as lower risk near threatened, 6 as vulnerable, 5 as endangered while 1 species was exotic.
https://jifro.areeo.ac.ir/article_117465_df634215663b22fa974ced39189408a7.pdf
Fish assemblage
Habitat ecology
Fish diversity
conservation
Himalayan river
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
15
29
10.22092/ijfs.2018.117512
117512
مقاله پژوهشی
Growth yield, carcass traits, biochemical and non- specific immune parameters in grey mullet, Mugil cephalus Linnaeus, 1758 under cyclic starvation and L- carnitine supplementation
paria Akbary
paria.akbary@gmail.com
1
university of tehran
The main objective for the present research is to assess effect of cyclic starvation and L- carnitine supplementation on growth (body final weight, hepatosomatic index (HSI), daily growth rate (DGI), feed conversion ratio (FCR) and voluntary feed intake (VFI)), body compositions (crude protein, crude lipid, ash and moisture), biochemical (plasma total protein, glucose, cholesterol, triglyceride and liver glycogen) and immunological (lysozyme activity) parameters in grey mullet. To satisfy foregoing end, number of 240 fish in weight (1.33±0.26 g) were randomly fell into four groups (three replicates, 20 fish per tank). Two groups were fed in daily manner and the other two ones were kept starved for 7 days once in 2 weeks. Two groups diets were fed with carnitine free basal diet and the remaining were fed with 800 mg carnitine kg-1 diet. Cyclic starvation led to significantly decreased body final weight, DGI, FCR, total protein, triglyceride and cholesterol. In contrary, no significant differences were found across all groups for FCR, HIS, glucose, liver glycogen and lysozyme concentrations. At the same time, combined effects of cyclic starvation and carnitine supplementation were manifested in DGI and VFI parameters. In light of the above results, it can be noted that in both feeding regimes, growth, feed utilization and carcass quality in grey mullet were increased upon adding 800 mg carnitine kg-1 diet
https://jifro.areeo.ac.ir/article_117512_c5b49c621693e55b185b881580749e9f.pdf
Mugil cephalus
cyclic fasting
L-carnitine
Growth performance
Cholesterol
lysozyme
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
30
47
10.22092/ijfs.2018.117849
117849
مقاله پژوهشی
Viral nervous necrosis (VNN) an emerging disease caused by Nodaviridae in aquatic hosts: Diagnosis, control and prevention: A review
Mohammad Jalil Zorriehzahra
zorrieh@yahoo.com
1
Milad Adel
miladadel85@yahoo.com
2
Maryam Dadar
dadar.m77@gmail.com
3
Sanna Ullah
sunyuop@gmail.com
4
Mohaddes Ghasemi
mohades@yahoo.com
5
Iranian Fisheries Science Research Institute (IFSRI)
Iranian Fisheries Science Research Institute (IFSRI)
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R. Iran.
Fisheries and Aquaculture Lab, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Iranian Fisheries Science Research Institute (IFSRI), Inland Water Aquaculture Research Center, Bandar Anzali, I.R. Iran.
Betanodavirus is one of the two genera making up the family Nodaviridae and is the etiological agent of viral nervous necrosis (VNN, also known as viral encephalopathy and retinopathy or VER). The virus infects a large range of host species in more than 50 species of marine and freshwater fish worldwide from different geographical areas and the known host range continues to expand as new species of fish are used for aquaculture. The disease is characterized by vacuolating necrosis of neural cells of the brain, retina and spinal cord and causes up to 100% mortality in larval and juvenile fish, and can cause significant losses in older fish. The lack of knowledge about control and prevention of the disease makes the problem serious and impedes development of management approaches. Therefore this review focuses on current knowledge and future perspectives of viral nervous necrosis in the aquaculture industry with special focus on the type of diagnosis, control and prevention of the disease.
https://jifro.areeo.ac.ir/article_117849_9fe4e931817ae6478d7e7a52d8405bbe.pdf
Viral Nervous Necrosis
Betanodavirus
Diagnosis
Control and prevention
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
48
59
10.22092/ijfs.2018.117623
117623
مقاله پژوهشی
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
Hamid Reza Alizadeh Sabet
h.alizadeh@modares.ac.ir
1
Mohammad reza Kalbassi
kalbassi_m@modares.ac.ir
2
Mohammad Pourkazemi
pourkazemi@ifro.ir
3
Majid Sadeghizadeh
sadeghma@modares.ac.ir
4
Iranian Fisheries Science Research Institute, Coldwater Fishes Research Center
Tarbiat modares university
Iranian Fisheries Science Research Institute
Tarbiat modares university
Caspian trout (Salmo trutta caspius Kessler, 1877) is an endemic migratory subspecies in the Caspian Sea, possessing ecologic-economic and biodiversity greatly value. Among habitat factors, temperature and its possible effects on the Caspian trout genome were considered in this research. During Embryonic developmental stages (fertilization–hatching), genomic alterations of single cells were assessed by Comet assay technique in three thermal treatments (4, 8, 12ºC). After artificial propagation of wild broodstocks, the batches of eggs (No. 500), were transferred to well equipped incubators with constant temperatures, water recycling systems by REDD(spawning area of trout)water, 3 stages water filtration (Si+Active Coal+Zeolite), UV-C application on effluent of each incubator and DO – pH – temperature digital controllers in 3 replicates. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis technique were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indexes (CometScore software ©2013 TriTek Corp) as DNA damage markers. Significant difference between thermal treatments (P<0.05) and comparison between mean values of comet TL index indicated 85.19 ±9.2 , 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT mean values measured as 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM mean values measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments respectively. Warm (12°C) treatments demonstrated greater DNA damages in comparison to the others. TM explained better information in comparison with TL & DT in all analyzed samples. In conclusion, temperatures out of optimal thermal conditions could significantly affect Caspian trout genome which could be subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.
https://jifro.areeo.ac.ir/article_117623_8a9897b7a2e82b0156a91c970e9178f9.pdf
Caspian trout
Salmo trutta caspius – Temperature – DNA damage- Comet assay
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
60
70
10.22092/ijfs.2018.116366
116366
مقاله پژوهشی
Stock identification of Arabian yellow fin Sea bream (Acanthopagrus arabicus) by using shape of otolith in the Northern Persian Gulf &Oman Sea
Mastooreh Doustdar
mastooreh.doustdar@gmail.com
1
Farhad Kaymaram
farhadkaymaram@gmail.com
2
Mahvash Seifali
masaifali@gmail.com
3
Shahla Jamili
shahlajamili45@yahoo.com
4
Ali Bani
ali_bani_2000@yahoo.com
5
Reaserch lecturer
Associate Professor
Assistant Professor
Associate Professor
Associate Professor
Analysis of the shape properties of fish otolith is one way to identify stocks of different species in the marine environment. Length, width, area, perimeter, form factor, aspect ratio, roundness, circularity, ellipticity and rectangularity analyses of otoliths were undertaken to assess patterns of spatial and temporal stock structure of a wide-ranging fish, the Arabian yellow fin sea bream Acanthopagrus arabicus. Fish were sampled from 125 stations across the distribution range of the species in the Northern Persian Gulf & Oman Sea from June 2014 to May 2016. Analysis of morphometric parameters of otolith showed the minimum size in Khuzestan waters and the West Strait of Hormuz and the maximum size in the East Strait of Hormuz. In Bushehr waters, the amount of form factor was in the lowest and circular factor showed the highest. These findings are in agreement with the most irregularity in otoliths margin of this area. The highest aspect ratio of otolith indicated the great growth in Khuzestan Waters. The thinner and longer otoliths were identified in the East Strait of Hormuz region. Further, rectangularity ratio factor in the Bushehr and West Strait of Hormuz waters was higher in comparison with other regions and this finding confirmed the quadrilateral otolith shape of this region. There are significant differences among otolith morphometric variables of the A. arabicus (P<0.05). The result of discriminant analysis on morphometric parameters of analysis on otolith morphometric parameters indicated that 53/8 percent were in their geographic location accurately.
https://jifro.areeo.ac.ir/article_116366_fcfbee40a7bd4b77fd0d8a0bb165d30b.pdf
shape otolith indices
Stock identification
Acanthopagrus arabicus
Northern Persian Gulf &
Oman Sea
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
71
82
10.22092/ijfs.2018.117478
117478
مقاله پژوهشی
Oral administration of garlic powder (Allium sativum) on growth performance and survival rate of Carassius auratus fingerlings
Shahram Dadgar
shdadgar@ifro.ir
1
M Seidgar
2
A Nekuiefard
3
A.R Valipour
4
M Sharifian
5
M Hafezieh
6
Iranian Fisheries Science Research Institute
National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Urmia, Iran
National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Urmia, Iran
Inland waters research center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Anzali, Iran
Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
This study was carried out to evaluate the effect of different levels of garlic (Allium sativum) on growth survival and nutritional characteristics of goldfish (Carassius auratus). For this purpose, 180 goldfish with an average initial weight of 1.18± 0.08 g were introduced randomly into 12 aquaria (50×30×40 cm) at 4 groups. After one week feeding on basal diet, the treatments were fed on experimental diets for 8 weeks. Garlic powder was added at different levels as follows: 0 (Control), 0.5, 1 and 1.5 mg/kg commercial diet. At the end of the experiment, growth, nutritional performance and survival were evaluated. There was no significant difference in growth performance and feed efficiency between treatments, although the growth and nutrition efficiency were better in 0.5% garlic powder treatment. The highest survival rate was achieved in the 0.5% garlic powder treatment that showed a significant difference comparing to control diet (p<0.05). The best growth performance and feed efficiency were obtained from fish fed by 0.5% garlic powder diet.
https://jifro.areeo.ac.ir/article_117478_b30e9dd945ad4efe72d5ab298bd8add2.pdf
Garlic powder
Growth
Feeding
survival
Carassius auratus
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
83
94
10.22092/ijfs.2018.117467
117467
مقاله پژوهشی
Investigation of physicochemical factors, chl-a and primary production in Mahshahr Creeks (Northwest Persian Gulf)
Negar Ghotbeddin
ghotbeddiny2005@gmail.com
1
Laleh Mosavi Dehmordi
2
Department of Fisheries, College of Agriculture and Natural Resources, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
Iran, Behbahan, Behbahan Khatam Alanbia University of Technology, faculty of Natural Resources, Department of Fisheries
The study on physiochemical factors and primary production of the aquatic ecosystems is important to know the characteristic of environment and capacity of recharge. Mahshahr creeks are the most important creeks in Khuzestan, Iran. In this study, variations in physiochemical factors, chl-a and primary production in 3 regions (Mahshahr, Zangi, and Moussa entrance creeks) were evaluated in 2 months of every season of 2014 in different depths (1m and 8m). The following variable has been analyzed: nitrate, phosphate, nitrite, silicate, temperature, salinity, DO, pH, conductivity, turbidity, light, chl-a, primary production and phytoplankton abundance. The maximum amount of chl-a (1.6 mg m-3) was observed in the shallow region of Mahshahr creek in April while the minimum amount (0.08 mg m-3) was seen in the depth region of Zangi in July. The highest (1.59 gc m-2 day-1) and lowest (0.02gc m-2 day-1) amount of primary production were observed in the shallow region of Moussa entrance in April and the depth region of Zangi creek in Nov, respectively. The maximum abundance of shallow and depth phytoplankton was observed in April and the minimum was observed in July in all of the three creeks. The results of correlation test between the studied factors revealed that primary production has a significant relationship with nitrite, chl-a, phytoplankton abundance and light. Primary production in these creeks is relatively high in April, but in other months the production is low.
https://jifro.areeo.ac.ir/article_117467_b64ed9df0b5735750bd4def3090c850c.pdf
Physiochemical factors
Chl-a
Primary production
Mahshahr Creeks
Persian Gulf
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
95
109
10.22092/ijfs.2018.117437
117437
مقاله پژوهشی
The effects of Rosmarinus officinalis essential oil on the quality changes and fatty acids of Ctenopharyngodon idella
laleh roomiani
l.roomiani@yahoo.com
1
mansoreh ghaeni
mansoreh.ghaeni@gmail.com
2
mahsa moarref
mahsamoarref@yahoo.com
3
Roxana Fallahi
4
Farahnaz Lakzaie
5
university
islamic azad university
Iranian Fisheries Science Research Institute, Tehran, Iran
Iranian Fisheries Science Research Institute, Tehran, Iran
The effects of the use of essential oil of Rosmarinus officinalis on the chemical quality and fatty acids of fish fillets of Ctenopharyngodon idella were investigated during frozen storage at -18 ºC for 6 months. Fish fillets were divided into three groups the control (C) without rosemary essential, the second group with 0.2 % rosemary essential oil (A) and the third with 0.4 % rosemary essential oil (B). According to compositional analysis of the rosemary essential oil by GC-MS, the α-pinene (31.91%) and 1, 8- cineol (14.66%) were the predominant components. Results showed that there were no significant differences of protein in group C and A, but were between A and B (P< 0.05). A significant decrease in lipid was obtained throughout between groups during storage (P< 0.05). There were no significant differences of pH between three groups at storage periods (P> 0.05). Effect of rosemary essential oil showed that the least change fatty acids were on polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA), respectively. Microbial results showed TVC content of fillets fish did not exceed the limit during storage period for A and B Groups (< 7 log CFU/g). Rosemary essential oil with 0.2% and 0.4 % was effective in controlling the chemical compositions and fatty acids, but sensory attributes reveals a decreasing trend in the attributes like color, odour, taste, firmness and general acceptance for two groups (P< 0.05), but group A indicated better scores than Group B at the end of the storage period.
https://jifro.areeo.ac.ir/article_117437_e54f575ce979903de7fd856f6c8613dd.pdf
Ctenopharyngodon idella
Rosmarinus officinalis essential oil
Chemical compositions
fatty acids
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
110
123
10.22092/ijfs.2018.117675
117675
مقاله پژوهشی
Effects of different cooking methods on minerals, vitamins and nutritional quality indices of grass carp (Ctenopharyngodon idella)
Sara Golgolipour
golgolipour@yahoo.com
1
Ainaz Khodanazary
khodanazary@yahoo.com
2
Kamal Ghanemi
ghanemi@yahoo.com
3
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marin Science and Technology, Khorramshahr, Iran.
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marin Science and Technology, Khorramshahr, Iran.
Department of Marine Chemistry, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
This study aimed to evaluate the nutritional value (proximate, fatty acid profiles, vitamins and mineral contents and also nutritional quality indices (NQI)) of grass carp (Ctenopharyngodon idella) prepared according to common consumer techniques: raw, poached, steamed, microwaved, pan-fried and deep-fried (in olive oil). In comparison to raw fish fillets, when grass carp was cooked there was an increase in protein, lipid and ash contents. Cooking methods had no significant effect on total n-3 fatty acids except for frying fillets. Lowest and highest content of n-3 was shown in deep-fried and pan-fried samples, respectively. Total n-6 fatty acid of cooked samples increased in comparison to raw samples. Na, K, Mg, P and Zn contents of boiled fish fillets significantly decreased. None of cooking methods had a significant effect a vitamin D. However, Vitamin A, B1 and B3 content of cooked fish significantly decreased.
https://jifro.areeo.ac.ir/article_117675_eba66ab5b7d9e8dad4e980d324f574ad.pdf
Cooking method
Grass carp
fatty acids
Vitamins
Minerals
Nutritional quality indices
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
124
139
10.22092/ijfs.2018.117600
117600
مقاله پژوهشی
Scale characteristics of the bloom event: A case study in the Iranian coastal waters of the Southern Caspian Sea
Asieh Makhlough
asieh_makhlough@yahoo.com
1
Hassan Nasrollahzadeh Saravi
hnsaravi@gmail.com
2
F Eslami
3
A.R Keyhansani
4
Ecology Deprt.
Ecology Deprt.
Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran
Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran
Nutrient enrichment in water and sediments due to excessive anthropogenic activities in recent years has caused excessive algal growth in the Caspian Sea. The current study was conducted to survey the abundance of phytoplankton, its dominant species and chlorophyll-a [Chl-a] concentration in algal bloom aspect in the Iranian coastal water of Caspian Sea during four seasons from 2013 to 2014. The minimum and maximum phytoplankton abundance was recorded 73±31 and 505±55 million cells/m3 in summer and winter, respectively. The median concentration of Chl-a was increased to 5.81 mg/m3 in autumn, as compared to annual median value (2.43 mg/m3). The result indicated that bloom started in autumn and it continued falling with a low rate during winter (Chl-a :2.59 mg/m3). The three species Stephanodiscus socialis, Binuclearia lauterbornii and Thalassionema nitzschioides classified in medium bloom class (100-1000 cells/mL) in spring, summer and autumn seasons, respectively. While in winter Pseudonitzschia seriata and Dactyliosolen fragilissima was classified in medium bloom class with high relative frequency. Finally, the bloom at the regions with more than 100 cells/m3 of total phytoplankton abundance and dominant species was overlapped with the bloom regions based on Chl-a concentration.
https://jifro.areeo.ac.ir/article_117600_a1a0281617d1b7181b11022be3010b3c.pdf
Phytoplankton
Bloom
Scale characteristics
Caspian Sea
Iran
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
140
162
10.22092/ijfs.2018.117727
117727
مقاله پژوهشی
Fish assemblage and structure as well as hydrological parameters at Karatoya Fish Sanctuary, Panchagarh, Bangladesh
Md. Rashidul Islam
mrislam_fbg@hstu.ac.bd
1
Hajee Mohammad Danesh Science &amp; Technology University, Dinajpur-5200, Bangladesh
Abstract Spatiotemporal variation in fish assemblage structure was conducted from January to December 2015 in order to know the impacts of sanctuary on ichthyo-faunal diversity with its indices and major hydrological factors from six sampling stations of Karatoya fish sanctuary sectioned in the river Karatoya. A total of 69 fish species was obtained from this sanctuary including 21 threatened species where Aspidoparia jaya, Pethia ticto, Puntius sophore, Canthophrys gongota and Barilius barna was the major contributory species (> 4.17%) both in space and time. The uppermost species richness and abundance were viewed in January and lowest in May. Based on analysis of similarities (ANOSIM), fish assemblages were significantly different equally in station (P < 0.001, R = 0.15) and month (P < 0.001, R = 0.62). Through two-dimensional nonmetric multidimensional scaling (nMDS) and cluster analysis based on Bray-Curtis similarity index, assemblages were alienated into two groups at a value of 42% and 28% partition for station and month, respectively. Canonical Correspondence Analysis (CCA) recognized considerable relations between a number of fishes and hydrological parameters where dissolved oxygen (mg/l) and water temperature (ºC) were the main leading factors in shaping fish assemblage structure.
https://jifro.areeo.ac.ir/article_117727_38301578821c50e9b558e7af254271f0.pdf
Fish assemblage
spatial and temporal variation
stream biodiversity
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
163
172
10.22092/ijfs.2018.117901
117901
مقاله پژوهشی
Effect of different levels of iron sulfate on some haematological parameters of ship sturgeon, Acipenser nudiventris
Hamid Hosseini
hamid.hosseini1982@googlemail.com
1
The present study was conduct to examine different dietary levels of iron (FeSo4) on some haematological parameters of Ship sturgeon, Acipenser nudiventris including red blood cells (RBC), white blood cells (WBCs), differential WBCs, hematocrit (Hct), Hemoglobin (Hb), the mean corpuscular hemoglobin (MCH) and the mean corpuscular hemoglobin concentration (MCHC), the mean corpuscular volume (MCV), immunoglobulin (IgM), ferritin, transferrin and plasma Fe2+. Four experimental treatments and one control group with three replicates were considered for the experiment. The experimental treatments were fish fed by experimental diets containing different levels of FeSo4 i.e. 100, 200, 250, and 300 mg/kg diet. After 60 days, there were significant differences between experimental groups in terms of WBCs, lymphocytes, neutrophils, monocytes, eosinophils, MCH and MCHC, MCV, total plasma protein, IgM, ferritin, transferrin and plasma Fe2+ (P<0.05). In 100 mg.iron/kg.diet treatment, more levels of IgM and total plasma protein were observed compared to other experimental groups (P<0.05). The highest values of neutrophils, monocytes and MCHC were found in control fish whereas MCV, MCH and eosinophils values were higher in 200 mg iron/kg.diet treatment (P<0.05). Also, more WBCs were observed in 250 mg.iron/kg.diet treatment than in other groups (P<0.05). The values of lymphocytes, RBC, Hct, Hb, ferritin, transferrin and plasma Fe2+ were higher in fish fed by 300 mg.iron/kg.diet compared to other experimental groups (P<0.05). In conclusion, our results showed that the supplementation of ship sturgeon diet with high levels of iron enhances the RBC, Hct, Hb, ferritin, transferrin and plasma iron.
https://jifro.areeo.ac.ir/article_117901_c04c8b15bae5c5be5898ed1afd77232c.pdf
iron sulfate (Feso4)
haematological parameters
Ship sturgeon
Acipenser nudiventris
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
173
187
10.22092/ijfs.2018.118806.
118009
مقاله پژوهشی
The synergistic influence of the sea cucumber (Holothuria arenicola) extract and dacarbazine on murine melanoma cancer cells B16F10
Javad Baharara
baharara78@gmail.com
1
Najme Nikdel
nikdelnajme@yahoo.com
2
Khadije Nezhad Shahrokhabadi
shahrokhabady@yahoo.com
3
Elaheh Amini
elah.amini73@gmail.com
4
Department of Development Biology, Research Center for Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Young Researchers and Elicit club, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Melanoma has been reported a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) have been examined on melanoma cells and compared with dacarbazine. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of methanolic SCE Also, several methods were exerted to detect cell dying by SCE and dacarbazine. The MTT assay showed that B16F10 cells proliferation were blocked by SCE (IC50=31µg/ml) and dacarbazine (IC50=1600 µg/ml) in a dose and time depended manner. Apoptosis induction yield treatment occurred in IC50 concentration of SCE and dacarbazine using DAPI staining, acridine orange/ propodium iodide, PI flow cytometry and annexin/PI assay. The caspase colorimetric kit indicated that SCE and dacarbazine could induce apoptosis through intrinsic pathway. The results suggested that the methanolic SCE has higher efficient cytotoxicity efficiency compared to dacarbazine. Therefore, SCE may be considered as a futuristic natural product regarding prevention or treatment of melanoma malignancy.
https://jifro.areeo.ac.ir/article_118009_b7048743971203573bbc528ae032819e.pdf
Apoptosis
natural product
melanoma
Cancer
dacarbazine
marine
caspase
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
188
198
10.22092/ijfs.2018.117890
117890
مقاله پژوهشی
Determination of some heavy metal levels in three freshwater fish in Keban Dam Lake (Turkey) for public consumption
Metin Caglar
firatmcaglar@gmail.com
1
Ozgur Canpolat
ocanpolat@firat.edu.tr
2
Zeliha Selamoglu
zselamoglu@nigde.edu.tr
3
Firat University
Fırat University
Nigde University
The accumulation of iron, zinc, copper and cobalt in the muscles of three freshwater fish (Cyprinus carpio, Squalius cephalus and Capoeta umbla) from Keban Dam Lake (Turkey) were investigated between September 2009 and April 2010. The concentration of heavy metals were found as Zn>Fe>Cu>Cd respectively. A positive relationship was found between heavy metal levels and fish size. The levels of all metals analysed in muscles of females were found to be higher than those in males. Our results showed that heavy metal levels which analyzed in this study in the muscles of three fish species from Keban Dam Lake were under the dangerous limits given by EPA and FAO and therefore there is no any risk for public consumption.
https://jifro.areeo.ac.ir/article_117890_d096a11e03197c498c337cdc1d4cfcd5.pdf
Heavy metals
Accumulation
Cyprinus carpio carpio
Leicuscus cephalus
Capoeta umbla Muscle
eng
Agricultural Research,Education and Extension Organization
Iranian Journal of Fisheries Sciences
1562-2916
2322-5696
2019-01-01
18
1
199
204
10.22092/ijfs.2018.116634
116634
مقاله پژوهشی
Zooplankton of Çat Dam Lake (Malatya-Turkey) with a new record for Turkish rotifers Lecane intrasinuata (Olofsson, 1917)
Serap Saler
ssaler@firat.edu.tr
1
Hilal Bulut
hilalhaykir@gmail.com
2
Gökhan Karakaya
gkarakaya23@gmail.com
3
Fırat University
Nineteen zooplankton species of them belonging to 15 Rotifera, 2 Cladocera, 2 Copepoda were recorded from Çat Dam Lake (Malatya). One of these species, Lecane intrasinuata (Olofsson, 1917) is a new record for Turkish rotifer fauna.
https://jifro.areeo.ac.ir/article_116634_ef988d37b4eb4f96f71425803c0f20d1.pdf
Rotifera
Lecane intrasinuata
new record
Turkey
Çat Dam Lake