ORIGINAL_ARTICLE
Effect of 2-phenoxyethanol anesthesia on some haematological and biochemical indices of silver carp (Hypophthalmichthysmolitrix)
In this study, the anesthetic effects of 2-phenoxyethanol (2-PE) on possible primary (cortisol level) and secondary (haematological indices and glucose level) stress responses were studied in silver carp (Hypophthalmichthys molitrix). Fish were first exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, also the time to induction (deep anesthesia) and recovery were measured. At concentrations of 0.5, 0.7 and 0.9 mL/L, all fish were anaesthetized within 3 min of exposure. For assessing possible stress effects caused by effective concentrations of 2-PE, the haematological indices, serum cortisol and glucose were determined in the deeply anaesthetized fish as stress indicators. 2-PE exposure resulted in a significant increase in red blood cell (RBC) amounts at 0.1 and 0.3 mL/L. It was observed a parallel increase in hemoglobin and Haematocrite amounts at 0.1 concentrations (P < 0.05). The serum cortisol levels had the highest amount in 0.1 mL/L of 2-PE. Moreover 2-PE exposure resulted in a significant increase in glucose amounts at the first 0.1 then 0.3 mL/L concentrations. This study shows that rapid induction of deep anesthesia with a relatively high concentration of 2-PE (0.5, 0.7 and 0.9 mL/L) was connected with the lowest effects on the haematological and serum biochemical indices in silver carp and so 0.7 mL/L could be suggested as suitable doses for haematological studies in this species.
https://jifro.areeo.ac.ir/article_115578_f68d7a09acfdc45473be6379daab82dc.pdf
2018-01-01
1
10
10.22092/ijfs.2018.115578
Blood parameters
Anesthesia
Stress response
Silver carp
Aliakbar
Hedayati
hedayati@gau.ac.ir
1
Scientific Board
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effect of Enriched Rotifer (Brachionus plicstilis) with Probiotic Lactobacilli on Growth, Survival and Resistance Indicators of Western White Shrimp (Litopenaeus vannamei) larvae
This study examined the effects of a commercial Lactobacillus probiotic on growth, survival and resistance of western white shrimp (Litopenaeus vannamei) larvae against salinity and formalin stresses in Persian gulf and Oman sea ecology research center. In this experiment, larvae were fed on 6 times a day from mysis I (M1) step to post larvae 5 over 3 treatments with 3 repetitions including a control treatment C (without probiotic) and 2 experimental treatments named A (having probiotic enriched rotifer) and B (having probiotic enriched rotifer and adding probiotic powder directly to the water) in 9 plastic tanks (20-liter) containing 10-l of seawater with concentration of 50 larvae per liter. At the end of this period biometric larvae were studied under salinity and formalin stresses. Results showed that used probiotic bacteria have so significant and positive effects on shrimps' resistance, survival and growth (P < 0.05) that growth and survival rate of larvae fed with A and B treatments are significantly higher than the control group (P < 0.05). the results of the stress tests, also, indicated that probiotic containing treatments (A and B) have significantly higher survival rates in salinity and formalin stresses than control treatment (P < 0.05). The highest rate of survival and resistance was observed in treatment B which was not statistically different from that of treatment A (P > 0.05). According to the results, that using probiotic powder is effective on growth, survival and resistance rate of larval and post larval steps of western white shrimp.
https://jifro.areeo.ac.ir/article_115581_b7394252743e8d6867f9883e37e44bfe.pdf
2018-01-01
11
20
10.22092/ijfs.2018.115581
Brachionus plicatilis
Probiotic
resistance
Litopenaeus vannamei
Nassim
Najmi
najmi.nassim@yahoo.com
1
Islamic Azad University
LEAD_AUTHOR
Maziar
Yahyavi
maziar_yahyavi@yahoo.com
2
Islamic Azad University
AUTHOR
A
Haghshenas
3
Shrimp Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bushehr, Iran
AUTHOR
ORIGINAL_ARTICLE
Health risk assessment of selected heavy metals in some edible fishes from Gorgan Bay, Iran
The objective of this study was to determine the distribution of selected metal concentrations (Cd, Pb, Cr and Fe) in the muscles of five fish species (Sander lucioperca, Liza auratus, Alosa caspia, Cyprinus carpio and Liza saliens) from Gorgan Bay the south-eastern Caspian Sea, in order to assess health risk to the human via fish consumption by graphite furnace atomic absorption spectrophotometry. Potential health risk assessments based on estimated daily intake (EDI) values and target hazard quotient (THQ) indicated that the intakes of metals by consuming these fish species do not result in an appreciable hazard risk for the human body. The hazard index (HI) calculated was lower than 1 for all the species. However, the results indicate that the concentrations high value of Pb (in the muscle of L. auratus) and Fe for all fish is alarming and do present an appreciable hazard risk for human health.
https://jifro.areeo.ac.ir/article_115582_15597c51d0c349ce8c5d861ebbd395bc.pdf
2018-01-01
21
34
10.22092/ijfs.2018.115582
Heavy metal
Fish
Gorgan Bay
EDI
THQ
Hossein
Alipour
hossein.alipour@yahoo.com
1
Young Researchers and Elite Club, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
LEAD_AUTHOR
Gholamreza
Banagar
gholam_banagar@yahoo.com
2
Department of Environmental Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
AUTHOR
ORIGINAL_ARTICLE
Effects of Probiotic, Pediococcus acidilactici, on Growth and Survival of Kutum, Rutilus kutum Fingerlings
In the present study effects of different levels of probiotic Pediococcus acidilactici, on growth performance and survival rate of Rutilus kuttum were investigated. Fingerlings Kutum with mean weight of 1±0.235 g were reared for 8 weeks in 500-l fiberglass tanks (15 fish per tank) with 4 diet treatments (with 3 replicates for each treatment). Treatments were included 1× 109 , 2:1×109 , 3× 109 CFU kg-1 dry food and without probiotic (control). At the end of the study, specific growth rate (SGR) and survival rate (SR) were higher in treatments supplemented with probiotic compared to control and its different were significant with together (p<0.05). Also, the fish fed with probiotics showed lowest food conversion ratio (FCR) significantly (p<0.05). The results of this study indicate that the use of 3× 109 CFU kg-1 dry food probiotics improved growth parameters and survival in Rutilus kuttum fingerlings.
https://jifro.areeo.ac.ir/article_115583_c71f52e29d11318776b69647850d3e50.pdf
2018-01-01
35
46
10.22092/ijfs.2018.115583
Rutilus kuttum
Probiotic
Pediococcus acidilactici
Growth
survival
Alireza
Valipour
valipour40@gmail.com
1
LEAD_AUTHOR
Najmeh
Hamedi Shahraki
razeqi8234@yahoo.com
2
AUTHOR
Hamid
Abdollahpour
abdollahpour51@yahoo.com
3
AUTHOR
ORIGINAL_ARTICLE
Population structure of banana shrimp, Penaeus merguiensis De Man, 1888 in Strait of Hormoz, Persian Gulf
Monthly length frequency data of banana shrimp, Penaeus merguiensis were collected from the coastal waters of North east Persian Gulf, from January 2012 to January 2013. Software package FiSAT was used to analyze the length frequency data. Growth parameters, mortalities, exploitation rate and recruitment pattern were estimated from the carapace length in the area. Based on length – weight relationship, the estimated parameters, a was 0.0015 for male and female and b was 2.8015 for male and 2.7852 for female. The estimated growth parameters: L∞=39.5 mm, K= 1.8 year−1 and t0=-0.08 year−1 for males and L∞ = 50 mm, K= 1.5 year−1 and t0=-0.09 year−1 for females. Longevity was estimated 1.67 and 2.00 years for male and female respectively. The estimated mortality coefficients, were Z=5.9 (4.89 – 6.92) year−1 (α=0.05), M=2.9 year−1 and F=3.0 year−1 for male and Z=5.7 (4.94 – 6.37) year−1 (α=0.05), M=2.5 year−1 and F=3.2 year−1 for female. The length at first capture, Lc estimated from the probability of capture routine was 23.3 mm for male and 24.2 mm for female. Sex ratio (M:F=1.1:1) was significantly different to 1:1. The exploitation rates of shrimp were 0.49 and 0.56 for male and female respectively which could get higher value because survival of spawning stock after fishing season related to environment factors have less effect on next year biomass. Recruitment was continued throughout the year but with one major peak in July to November.
https://jifro.areeo.ac.ir/article_115584_60b046116dea350f88689608f4297665.pdf
2018-01-01
47
66
10.22092/ijfs.2018.115584
Penaeus merguiensis
population dynamics
Persian Gulf
Hormozgan
Mohammad
Momeni
msmk63@yahoo.com
1
Persian Gulf &amp;amp; Oman Sea Ecology Research Institute
AUTHOR
Ehsan
Kamrani
ezas47@gmail.com
2
Hormozgan
LEAD_AUTHOR
Mohsen
Safaie
msn_safaie@yahoo.com
3
Hormozgan university
AUTHOR
Farhad
Kaymaram
farhadkaymaram@gmail.com
4
ifro
AUTHOR
ORIGINAL_ARTICLE
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases of farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon province of the Black Sea region of Turkey during field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and, compared them with 25 previously published gene sequences. Based on complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which closely related to GE-1.2 isolate (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago. These isolates could be an indigenous type of VHSV distributed in Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulating with indigenous strains which can threaten the aquaculture industry in Turkey.
https://jifro.areeo.ac.ir/article_115585_78e2e2f2be0129d96ffd3d84b26eb24a.pdf
2018-01-01
67
73
10.22092/ijfs.2018.115585
genotype
Rainbow trout
Turbot
Turkey
VHSV
Harun
ALBAYRAK
harun.albayrak@omu.edu.tr
1
Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
LEAD_AUTHOR
H
Isidan
2
Department of Virology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey
AUTHOR
G
Kalayci
3
Department of Virology, Bornova Veterinary Control Institute, İzmir, Turkey
AUTHOR
E
Ozan
4
Department of Virology, Veterinary Control Institute, Samsun, Turkey
AUTHOR
V.N
Vakharia
5
Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD, 21202, USA
AUTHOR
ORIGINAL_ARTICLE
Essential fish habitats (EFH) of small pelagic fishes in the north of the Persian Gulf and Oman Sea, Iran
Small pelagic fishes particularly anchovy (Encrasicholina punctifer) and sardine (Sardinella sindensis) have an important role to support the Iranian fisheries, and are distributed along the entire length of the Persian Gulf and Oman Sea coastal waters. Using small pelagic fisheries logbook, a GIS-based environmental modelling approach was applied to investigate the presence and abundance of anchovy and sardine in relation to environmental variables. Sea Surface Temperature(SST), Sea surface chlorophyll-a concentration (Chlo-a), Sea Surface Salinity (SSS), Photosynthetically Active Radiation(PAR), Wind Speed (WS), Wind direction (WD), Bathymetry (depth), Distance from the nearest coast and Sea ground slope were collected from internet-based sources and scientific documents monthly from October 2014 to March 2015. Redundancy analysis (RDA) was applied to provide preliminary view of relationships between fish presence/absence and environmental variables, followed by application of Generalized Additive Models (GAMs). GAMs indicated the presence/absence of fish was related to distance from nearest coast, depth, sea surface Chlorophyll-a and SST. Essential fish habitat (EFH) maps were generated for anchovy and sardine, based on the predicted probability of presence of each spices. Results of the EFH showed that sardine to be concentrated in specific areas of more favorable conditions, such as north of the Persian Gulf zone and all areas of north west of Oman Sea zone. However, EFH of anchovy showed a more widespread distribution, occupying most of the north west of Oman Sea coastal waters , south of Qeshm Island in Strait of Hormoz zone as well as Parsian district in north of the Persian Gulf zone. In this study it seems that the anchovy have probability presence whispered to the areas with more distance from the coastal waters. However, the EFH probability presences of sardine were predicted for near coastal water and obviously, shallower waters. Due to small pelagic fisheries development, it is highly recommended to investigate anchovy and sardine fishing possibility in areas with high EFH prediction probability. However, in order to increase the accuracy and scope of essential fish habitat prediction probability for small pelagic fishes, implementation of a comprehensive acoustic survey in small pelagic fishing season and fishing grounds were highly recommended for all Iranian coastal wateres.
https://jifro.areeo.ac.ir/article_115586_ced1b8dfd116d5ab063f3fbc7a2aed90.pdf
2018-01-01
74
94
10.22092/ijfs.2018.115586
Small pelagic fishes Essential Fish Habitat
Marine environment
Persian Gulf
Oman Sea
Ali
Salarpouri
salarpouri@pgoseri.ac.ir
1
Bandar Abbas, Iran. P.O. Box; 3955
AUTHOR
Ehsan
Kamrani
ezas47@gmail.com
2
Bandar Abbas, Iran. P.O. Box; 3955
LEAD_AUTHOR
Farhad
Kaymaram
farhadkaymaram@gmail.com
3
Tehran. Iran. P.O.Box; 149-14965
AUTHOR
Rasool
Mahdavi Najafabadi
ra_mahdavi2000@yahoo.com
4
Bandar Abbas, Iran. P.O. Box; 3955
AUTHOR
ORIGINAL_ARTICLE
Effects of Satureja bachtiarica and Nigella sativa essential oils on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss)
Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of Satureja bachtiarica and Nigella sativa essential oils on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean average of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection, vaccine only, DMSO injection, vaccine with 50, 100, and 200 microgram ip injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. At the sixth week, 1.7×107 cfu as LD50 96 hrs of Lactococcus garvieae were i.p injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing of the N. sativa essential oil concentration, with a significant difference with control groups (P<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate, although S. bachtiarica was not effective on immune parameters of the studied fish.
https://jifro.areeo.ac.ir/article_115587_9c2937b16e648d1664e3135f314314be.pdf
2018-01-01
95
106
10.22092/ijfs.2018.115587
Nigella sativa
Satureja bachtiarica
streptococcosis
lactococcosis
Adjuvant
Mehdi
Raissy
mreissy@yahoo.com
1
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
LEAD_AUTHOR
Saeed
Hashemi
hashemi.saeid72@yahoo.com
2
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
AUTHOR
Milad
Roushan
roushan7394@yahoo.com
3
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
AUTHOR
Mohsen
Jaafarian
mjafariand@yahoo.com
4
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
AUTHOR
Hassan
Momtaz
hamomtaz@yahoo.com
5
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
AUTHOR
Mehdi
Soltani
msoltani@ut.ac.ir
6
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
AUTHOR
Esmaeil
Pirali Kheirabadi
esmaeil_pirali@yahoo.com
7
Department of Fisheries Science, College of Natural Resources and Earth Sciences, University of Shahrekord, Shahrekord, Iran.
AUTHOR
ORIGINAL_ARTICLE
A comparative study on morphometric and meristic characters of Nemipterus japonicus (Bloch, 1791) in the coasts of India
A total of 200 threadfin bream Nemipterus japonicus was collected from Chennai in the east coast and Kochi in the west coast during January to February 2013 and studied for their morphometric and meristic characters. In total, 21 characters were analyzed out of which 3 characters namely dorsal, ventral and anal fins were not considered since they were unaltered. The findings indicate that 91% of the total variation in the data can be accounted for by three clusters. Small values of 1-R**2 ratio for the three clusters obtained indicate good clustering. The small values of P (P≤0.0001) indicate that there is high significant difference in the variables of Chennai and Kochi data which are shown through MANOVA. This might depict that the fish of Chennai and Kochi might be of 2 different stocks. Moreover, the study has also aided in identifying the importance of regular monitoring of stock assessment and the biology of commercially important fishes. A total of 200 threadfin bream Nemipterus japonicus were collected from Chennai in the east coast and Kochi in the west coast and studied for their morphometric and meristic characters. In total, 21 characters were identified and analysed out of which 3 characters namely dorsal, ventral and anal fins were not considered since they were unaltered. The study reveals a significant difference in the characters between the Chennai and Kochi samples, thereby depicting that the fish might be of 2 different stocks. Moreover, the study has also aided in identifying the importance of regular monitoring of stock assessment and the biology of commercially important fishes.
https://jifro.areeo.ac.ir/article_115588_a0824a8ace8386c8b888850191bfb3f0.pdf
2018-01-01
107
117
10.22092/ijfs.2018.115588
Nemipterus japonicus
Threadfin bream
Biometric study
Coastal area
India
morphometric
merisitic characters
Edwin
Prabakaran
teprabakaran@yahoo.com
1
Loyola College
AUTHOR
Jeyasingh
Thompson
jeyasinghthompson.r@gmail.com
2
Madras Christian College
LEAD_AUTHOR
Deepak
Samuel
deepakocean@gmail.com
3
UNDP, Gulf of Mannar Biosphere Reserve Trust
AUTHOR
ORIGINAL_ARTICLE
Changes in length-weight relationship and condition factor of Talang queenfish (Scomberoides commersonnianus) in the north-west Persian Gulf
Abstract Scomberoides commersonnianus is one of the commercially important fishes of the family Carangidae. In this study, 563 specimens (292 males, 247 females, and 24 sexually unknown ones) were collected from the north-west of the Persian Gulf. Length-weight relationships and condition factors were determined for each sex in four length classes within different seasons. In general, the growth pattern in this fish was negatively allometric and the length-weight relationship in all specimens amounted to W= 2.9109FL-1.8424. The growth pattern of all specimens was negatively allometric in spring and autumn and isometric in summer and winter. Also, growth pattern was positively allometric in 8-31 class, negatively allometric in 31-54 cm and 54-77 cm classes and isometric in 77-100 cm class among all specimens. The condition factor in the 8-31 cm, 31-54 cm and 54-77 cm length classes was greater in males than in females, but in 77-100 cm length class it was more in females than in males, which is probably due to the increase in ovary weight in this class. Also, the condition factor in males exceeded that of females in spring, autumn and winter however, it was greater in females than in males during summer. In this season, females were in better condition owing to an increase in the weight of ovaries. The results demonstrate that growth pattern and condition factor vary in different seasons, lengths and sexes because of change in feeding intensity and the time of reproduction.
https://jifro.areeo.ac.ir/article_115589_469f20c68c1d29743464fe492dd8b8c2.pdf
2018-01-01
118
136
10.22092/ijfs.2018.115589
Scomberoides commersonnianus
length-weight relationship
Condition factor
Persian Gulf
Seyyedeh Zahra
Masoomizadeh
zmasoomi@yahoo.com
1
Shahid Beheshti University
LEAD_AUTHOR
Jamileh
Pazooki
pazooki2001@sbu.ac.ir
2
Shahid Beheshti University
AUTHOR
Tooraj
Valinassab
t_valinassab@yahoo.com
3
Iranian Fisheries Research Organization
AUTHOR
ORIGINAL_ARTICLE
Feeding habits and trophic level of sharpnose stingray, Himantura gerrardi (Elasmobranchii Dasyatidae) in northern Oman Sea: effects of sex and size class
The feeding habits and trophic level of Himantura gerrardi were studied according to sex and size classes utilizing 80 individuals obtained using bottom trawl between May and December 2014 from Oman Sea. Collected specimens were divided in three size classes (small 58 cm) based on disk width (DW). Preys were identified in seven main zoological categories, where crustacean and polychaetes were the dominant in diets, which indicating this species is a demersal dwelling species. No significant differences were observed between sexes, but size classes showed a significance differences in their diets. Also, results showed a decreasing of feeding overlap between size classes by increasing of DW, suggesting that intraspecific competition reduced, which allows the coexistence of different size classes. Trophic level was positively correlated with the DW, which were lower than 4.0 for both sexes and different size classes, placing it in intermediate trophic level.
https://jifro.areeo.ac.ir/article_115590_95a011baa1d2bc575d068d9f0bde429f.pdf
2018-01-01
137
150
10.22092/ijfs.2018.115590
Dasyatidae
Himantura gerrardi
Feeding habits
Trophic level
Oman Sea
Ali Reza
Rastgoo
rastgoo.alireza@yahoo.com
1
Department of Marine Biology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
LEAD_AUTHOR
S.M.R
Fatemi
2
Department of Marine Biology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran.
AUTHOR
T
Valinassab
3
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), P.O. Box: 14965/149. Tehran, Iran.
AUTHOR
M.S
Mortazavi
4
Persian Gulf and Oman Sea Ecological Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran.
AUTHOR
ORIGINAL_ARTICLE
Molecular Diversity of Symbiodinium spp. within six coral species in Larak Island, the Persian Gulf
Reef- building coral harbor communities of photosynthetic taxa of the genus Symbiodinium (zooxanthellae). The genus Symbiodinium is currently classified into nine genetic clades (A–I). Various corals harbor different Symbiodinium clades some show specificity to single strain. Coral and their zooxanthellae are sensitive to environmental stresses. In the Persian Gulf, coral reefs are subject to harsh environmental conditions including extreme temperatures and high salinity. It is the first study to use clade specific primers to clarify the diversity of Symbiodinium in each coral species of Larak Island. For this purpose six coral species were collected at two different locations in Larak Island. After DNA extraction, PCR amplification was performed using clade specific primers. The results showed that multiple Symbiodinium clades are hosted by the most coral species. In addition, among thirteen obtained Symbiodinium sequences, the frequency of either tree clades, A, C and D was almost the same. Corals species may contain different clades of Symbiodinium depending on the region and on the tolerance characteristics of each clade. Thus, knowledge of zooxanthellae diversity associated with scleractinian can contribute to a better understanding of coral’s sensitivity to environmental condition.
https://jifro.areeo.ac.ir/article_115591_0e543f5f52bdb9250743ba82b71185ae.pdf
2018-01-01
151
161
10.22092/ijfs.2018.115591
Persian Gulf
Symbiodinium
clade A
clade C
clade D
Hamed
Dehghani
hamed.marine@yahoo.com
1
Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Pargol
Ghavam Mostafavi
gh.mostafavi@gmail.com
2
Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
LEAD_AUTHOR
Seyed Mohammad Reza
Fatemi
reza_fatemi@hotmail.com
3
Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Jalil
Fallah Mehrabadi
jalil.fallah@gmail.com
4
Department of Biology, School of Basic Science, University of Qom, Qom, Iran
AUTHOR
ORIGINAL_ARTICLE
Comparison of antibiotics and bacteriocins antibacterial activity on Xanthomonas citri subsp.citri
Citrus canker is a citrus disease species that created by the bacterium Xanthomonas citri subsp.citri. Many citrus, such as oranges, lime, and grapefruit are affected by the infectious bacteria and stems, leaves and fruits are experiencing loss. In this experiment antibacterial effects of five kinds of antibiotics including ampicillin, kanamycin, chloramphenicol, penicillin, streptomycin, Cronobacteriocin DGH2 and Enterobacteriocin DGH4 were evaluated on 107 strains of Xanthomonas citri subsp.citri. MIC and MBC data for antibiotics and bacteriocins against Xanthomonas strains were performed. According to this project, Xanthomonas strains were comparatively susceptible and resistant to Cronobacteriocin DGH2, Enterobacteriocin DGH4, ampicillin, kanamycin, chloramphenicol, penicillin and streptomycin. NIGEB-183 strain is most sensitive to these antibiotics and bacteriocins, on the other, only NIGEB-242R1 strain is resistant to chloramphenicol. Penicillin is also minimal inhibitory effects on Xanthomonas strains. Based on this case study, chloramphenicol is most antibacterial activity among of antibacterial agents and this agent is a good candidate for inhibitory activity. Cronobacteriocin DGH2 has a moderate antibacterial activity against Xanthomonas strains.
https://jifro.areeo.ac.ir/article_115592_f4f1ea0e0a4db0cc7b0cc220c43dfae2.pdf
2018-01-01
162
178
10.22092/ijfs.2018.115592
Antibiotics
Bacteriocins
Citrus canker
MIC and MBC data
Xanthomonas citri subsp.citri
Dariush
Gholami
1
AUTHOR
Saeed
Aminzadeh
aminzadeh.saeed@yahoo.com
2
NIGEB
LEAD_AUTHOR
Seyed Mehdi
Alavi
3
AUTHOR
Nasrin
Kazemipour
4
AUTHOR
Ahmad
Ghoroghi
5
AUTHOR
Z
Emruzi
6
Department of Bioprocess Engineering, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Trophic Level, Food Preference and Feeding Ecology of Rachycentron canadum (Linnaeus. 1766), in Hormuzgun province Waters (Northern Persian Gulf and Oman Sea)
The aim of this study was to assess the ecological factors associated with feeding by Rachycentron canadum (cobia) in the waters of Hormuzgan Province, Iran. Specimens were collected seasonally from Sep. 2014–Sep. 2015 from the Ferdows-1 survey ship and from landing areas in the city of Bandar Abbas. A total of 577 cobia were collected (340 male, 237 female) (20–161 cm Total length). Our results provide the first estimates of trophic level (TL) (4.4 ± 0.76, mean ± SE), consumption per biomass (Q/B) (6.13), and aspect ratio of the caudal fin (ARc.f) (3.1) for R. canadum in the Hormuzgan Province. We also found that osteichthies made up more than 80 percent of the relative importance of particular prey (IRI %), indicating that R. canadum primarily preys on these species. The immature group tended to feed on shrimp, crabs, and mollusks, and predominance of shrimp, crabs, and mollusks was also observed among the stomach contents of cobia ranging from 20–40(c.m). Hierarchical clustering revealed that the frequencies of different types of prey could be used to divide the length classes into three groups. A compare means test comparing the immature and mature groups and a one-way analysis of variance (ANOVA) comparing length classes revealed that the immature group and the 20-40 length class tended to feed on shrimp, crabs, and mollusks. Also, we found that males generally fed more than females in all seasons and that R.canadum had an average dietary intake. Our results suggest that the removal of top predators, such as cobia, could disturb the entire ecosystem because they play a fundamental role in maintaining a balance in the populations of their prey. Also, because diet is important for aquaculture, the findings of this study can be used to prepare a food formulation for cobia that resembles the native diet.
https://jifro.areeo.ac.ir/article_115608_d63aad0e7354d5fa10da4de35b9d183d.pdf
2018-01-01
179
193
10.22092/ijfs.2018.115608
Rachycentron canadum
Feeding Ecology
Trophic level
Consumption per biomass Persian Gulf and Oman Sea
Siamak
Behzadi
behzadi@pgoseri.ac.ir
1
Persian Gulf Oman Sea Ecological ResearchInstitute
AUTHOR
Ehsan
Kamrani
ezas47@gmail.com
2
Hormozgan
LEAD_AUTHOR
Farhad
Kaymaram
farhadkaymaram@gmail.com
3
ifro
AUTHOR
Mohammad Sharif
Ranjbar
sharif.ranjbar@gmail.com
4
AUTHOR
ORIGINAL_ARTICLE
Age, growth and spawning season of Luciobarbus esocinus Heckel, 1843 in Gamasiab River, Iran
In current paper reproductive strategy and growth rates of Luciobarbus esocinus were investigated from 2010 to 2012, in Gamasiab River, Kermanshah, Iran. Samples were collected seasonally by gillnet and electro-fisher device. Sex ratio was evaluated from 162 individual and the percentage of male and female individuals were 54.76% and 45.24% respectively. Age structure was distributed between I and V and most frequency numbers for males and females belonged to II and IV age group, respectively. Length-weight relationship was computed as W = 8E-05TL2.6546 for all individuals. Mean total length and weight was 333.82±13.10 mm and 297.67±4.85 gr for males, and 371.5±21.17 mm and 307.85±7.64 for females. The von-Bertalanffy growth models of L. esocinus were described as Lt =357.14×[1-e (-0.77× (t –0.090))] for males, Lt =367.02×[1-e (-0.78× (t –0.094))] for females and Lt =363.57×[1-e (-0.76× (t –0.087))]for both sexes. Results indicated that growth factors were highly similar in spring, summer and winter. CDA analysis revealed that the total and gonad weight are the main factors in discriminant analysis. The highest GSI values were found in spring for females and winter for males, however, it decreased rapidly after spring in females. Maximum growth rates observed in early ages (I-II) and had depletion with ages. K value and L∞ was determined 0.77±0.09 Y-1 and 357.14±10.38 mm for males and 0.78±0.24 Y-1 and 367.02±24.15 mm for females respectively. In conclusion this study provided basic information for stock assessment and management of L. esocinus in Gamsiab Reservoir.
https://jifro.areeo.ac.ir/article_115610_bcda09d3f92f14fce92732ce97068c8e.pdf
2018-01-01
194
207
10.22092/ijfs.2018.115610
Life-history styles
Reproduction
Luciobarbus esocinus
Iranian inland waters
Michael
Hajiahmadian
1
Natural resources faculty, Urmia University, Urmia, Iran
AUTHOR
Mahnaz
Rabbaniha
rab.mahnaz@gmail.com
2
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Tehran- Iran
AUTHOR
Hamed
Ghafari Farsani
hamed_ghafari@alumni.ut.ac.ir
3
Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
AUTHOR
Mohammad Hasan
Gerami
m.h.gerami@gmail.com
4
Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran
LEAD_AUTHOR
Saeid
Shahbazi Naserabad
5
Young Researchers and Elite Club, Yasuj Branch, Islamic Azad University, Yasuj, Iran
AUTHOR
ORIGINAL_ARTICLE
Meristic variations and skeletal deformities in natural population of mahseer fish, Tor putitora (Hamilton, 1822)
Skeletal deformities seriously relegate the biological performance of fish. The frequency of occurrence of different types of meristic variations and skeletal deformities were shown in population of Mahseer fish, Tor putitora, in two main reservoirs; Korang stream, Islamabad and Dhobi Ghat stream, Hassan Abdal. Total of ninety-five fishes were caught randomly, forty-five from the unpolluted Korang stream and fifty from the polluted Dhobi Ghat stream by cast nets from February to July, 2009. Radiographs and dry skeletal preparation techniques were used to record meristic variations and skeletal deformities and describe various types and frequency of appearance. Deformities were studied under categories, Highest Frequency of Occurrence (HFO), Normal Frequency of Occurrence (NFO) and Lowest Frequency of Occurrence (LFO). In the deformed fishes, significant (p<0.001) anomaly was noticed in nineteenth to twenty second and forty first to forty third (p<0.05) vertebrae of the vertebral column. Deformities scoliosis (C2, 5.26%), platyspondyly (C5, 11.27%), and abnormal neural spine (TC25, 13.53) were appeared to affect 70%, 60% and 50% of the deformed individuals, respectively. This study concluded that skeleton deformities in Tor putitora population in laundry waste water of Dhobi Ghat stream have relevance with detergent waste, as possible causative agent.
https://jifro.areeo.ac.ir/article_115612_fff5485783fdc51adfa40967ca7aa318.pdf
2018-01-01
208
216
10.22092/ijfs.2018.115612
Meristic variations
Skeleton
Deformities
Tor putitora
Zahid
Majeed
zahidfdb@gmail.com
1
Qauid-i-Azam University
LEAD_AUTHOR
Zainab
Ajab
2
xx
AUTHOR
Amina
Zuberi
zuberi_amina@yahoo.com
3
xx
AUTHOR
Shamim
Akhtar
4
xx
AUTHOR
Ali
Muhammad
5
xx
AUTHOR
ORIGINAL_ARTICLE
Effects of dietary diludine supplementation on growth, proximate composition, muscle and texture structure of rainbow trout juveniles
A feeding trial which lasted for eight weeks was conducted to investigate the effects of diludine such as growth promoter on feed efficiency, muscle structure and proximate composition of juvenile rainbow trout. Diludine was added at 0.0(D0) 0.2(D1), 0.5(D2) and 1(D3) g kg-1 to a casein-based diet, and every diet was given to the triplicated groups of juvenile rainbow trout. At the end of experiment, it was determined that a significant improvability existed for both growth and feed utilization in fish fed diets supplemented with diludine (p < 0.05). Similarly, different concentration of diludine affected the densitometric quantification of myofibrillar proteins in fish muscle according to results obtained by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The mean value of fiber diameters significantly increased in skeletal muscle by means of the increasing concentration of diludine. The histological results also showed hypertrophic adipocytes in skeletal muscle of fish fed D2 and D3 diets. The lowest elasticity values were observed in fish fed control diet while those fed D3 diet had highest elasticity values. On the other hand, no differences were found between fish fed experimental diets in terms of survival rate and all fish exhibited similar proximate composition for protein, lipid, moisture and ash. Consequently, it may be suggested that dietary diludine supplementaion up to 1 g kg-1 concentration in the diets have positive impacts on growth of rainbow trout juvenils and the better growth in the fish fed with diludine supplements could be arise from muscle characteristics, in particular changes in fibres than proximate composition of the muscles.
https://jifro.areeo.ac.ir/article_115616_c95c8543f49f15b95d0f4e8323679426.pdf
2018-01-01
217
227
10.22092/ijfs.2018.115616
Diludine
Growth
Muscle structure
Texture
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
trout
O
Hisar
1
Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, Çanakkale-Turkey
AUTHOR
M.M
Karaoğlu
2
Faculty of Engineering, Department of Food Engineering, Atatürk University, Erzurum- Turkey
AUTHOR
S.A
Hisar
3
Faculty of Engineering, Department of Food Engineering, Çanakkale Onsekiz Mart University, Çanakkale-Turkey
AUTHOR
Telat
Yanık
talatyanik@yahoo.com
4
Atatürk University
AUTHOR
M
Gürkan
5
Faculty of Art and Science, Department of Biology, Çanakkale Onsekiz Mart University, Çanakkale-Turkey
AUTHOR
Hasan
Kaya
6
Çanakkale Onsekiz Mart University
AUTHOR
ORIGINAL_ARTICLE
Evaluation of ecological status of the Persian Gulf inshore waters (Hormozgan rocky bottoms) using macrophytic communities and a macroalgae biological index, EEI
Abstract: Marine macro-algae are very important biological indicators of the ecological status of the marine environment because they are first to react to the pollution entering the sea including nutrients which determine the trophy of the ambient of the environment. The aim of this paper is to evaluate the present trophic level and the health status of the sampling stations according to the European Water Frame Directive. Sampling was done bi-monthly at seven stations at the tidal rocky shores west of Hormozgan Province. In this study a total of 63 of species were identified, from which 15 species, seven genes belonged to green algae16 species, five genes belonged to brown algae, 32 species, nine genes belonged to red algae The values of abundance of macroalgae and Ecological Evaluation Index indicate a high level of eutrophication and bad status for the Station 1 and poor status for Station 2, Station 3, Station 4 and Station 6. They are classified as zones with a bad and poor ecological status. It has been proved that chlorophyll and nutrients are higher in these zones. This fact just confirms the results obtained from macroalgae metrics. The best values of the estimated metrics at Station 5 and Sation 7 could be explained with the good and moderate ecological conditions in that zone and the absence of pollution sources close to those stations.
https://jifro.areeo.ac.ir/article_115619_accf4a4870f641c612b5f3ecc767cedb.pdf
2018-01-01
228
238
10.22092/ijfs.2018.115619
Key words: Ecological Evaluation Index
Trophic Index
Water Framework Directive
Macroalgae
Zahra
Alavian
z_alavian@yahoo.com
1
Department of Marine Biology, Science and Research Branch, Islamic Azad University
AUTHOR
H
Riahi
h-riahi@sbu.ac.ir
2
Department of Biology, Science Branch, Shahid Beheshti University, Tehran, Iran.
AUTHOR
rezvan
mousavi nadushan
mousavi.nadushan@gmail.com
3
Azad University
LEAD_AUTHOR
B
Reeisi
4
Department of Mathematic, Shahed University, Tehran, Iran
AUTHOR
S.M.R
Fatemi
5
Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
AUTHOR
ORIGINAL_ARTICLE
Short communication: Identification of By-catch species of tuna purse seiners in Iranian waters of Oman Sea
Tuna purse seine fishery is a commercial and industrial fishery in the Iranian waters of Oman Sea. An observer programme was conducted to identify the by-catch composition of the tuna purse seine fishery in the Iranian waters of the Oman Sea. Investigations were carried out on board of purse seiner vessel “named Parsian Shila” between September and October 2015. The by-catch was composed of 17 species belonging to 13 families. The composition of by-catch species included: Auxis thazard (22.79%), Coryphaena hippurus (11.4%), Istiophorus platypterus (12.66%), Makaira indica (5.07%), Sphyraena putnamiae (5.07%), Sphyraena jello (1.26%), Echeneis naucrates (2.54%), Elagatis bipinnulata (3.8%), Naucrates doctor (1.26%), Selar crumenophthalmus (1.26%), Rhizoprionodon acutus (24.05%), Rhincodon typus (1.26%), Alopias pelagicus (1.26%), Gymnura poecilura (1.26%), Mobula diabolus (1.26%), Aetobatus narinari (1.26%) and Chelonia mydas (2.54%). Bony fish with 67%, elasmobranches with 30% and turtles with 3% constituted the total by-catch individual. Results of this study provide valuable information in order to manage reduction of by-catch in tuna fisheries by purse seiners in Iranain waters of Oman Sea.
https://jifro.areeo.ac.ir/article_115626_96e06df0cdb052d5e69f3910eb0d459c.pdf
2018-01-01
239
243
10.22092/ijfs.2018.119084.
By-catch
Tuna
Purse Seine
Oman Sea
Mehran
Parsa
1
Hormozgan University
AUTHOR
Ehsan
Kamrani
eza47@gmail.com
2
Hormozgan University
LEAD_AUTHOR
Mohsen
Safaie
msn_safaie@yahoo.com
3
Hormozgan University
AUTHOR
Seyed Yousef
Paighambari
sypaighambari@yahoo.com
4
Gorgan University of Agricultural Sciences and Natural Resources
AUTHOR
T
Nishida
5
National Research Institute of Far Seas Fisheries, Fisheries Research Agency, Shimizu, Shizuoka, Japan
AUTHOR
ORIGINAL_ARTICLE
Short communication: Study on bioaccumulation of heavy metals (cadmium, nickel, zinc and lead) in the muscle of wels catfish (Silurus glanis) in the Anzali Wetland
Catfish of Anzali wetland has been focused economically and commercially. It also meets the religious Minorities needs to fish meat. So, investigation of its health in the wetland is important. This study aimed to investigate the concentration of heavy metals such as cadmium, Nickle, Zinc and lead in muscle tissues of Catfish in three stations (west, central and east) of Anzali wetland in 2012. In this study, 10 samples having standard weights were selected randomly from each station by fishing net. The atomic absorption set was used to measure the concentrations of heavy metals. The results showed that the average concentration of cadmium in Catfish was 0.05 and 0.02 microgram / dry weight in west and central wetland, respectively. This was not distinguishable in the east wetland by set. It was found that the average concentrations of Nickle in S.Glanis were 0.18 , 0.1 and 0.09 microgram /gr dry weight in west, central and east wetlands, respectively. Also, the average concentrations of zinc in S.Glanis were 26.35, 20.25 and 22.3 microgram /gr dry weight in west, central and east wetland, respectively. The average concentrations of lead in Catfish were 0.49, 0.5 and 1.11 microgram /gr dry weight in the west, central and east wetland, respectively. In this study, concentrations of cadmium, Nickle, zinc and lead in Catfish were not significant among the studied stations (P > 0.05). Cadmium, Nickle, Zinc and lead levels were compared with standards determined by food and Drug of America (FDA) and world health organization (WHO). Results showed that levels of Cd, Ni, Zn toxicities were fewer than the standards reported by FDA and WHO. However Pb concentration was observed more than the allowed limit (WHO = 0.5) in the east wetland, this needs more investigations.
https://jifro.areeo.ac.ir/article_115628_17327930b235d08a5cde787074b8c613.pdf
2018-01-01
244
250
10.22092/ijfs.2018.118782.
Catfish
Anzali Wetland
Heavy metals
Muscle tissue
aliasghar
khanipour
aakhamipour@yahoo.com
1
LEAD_AUTHOR
M
Ahmadi
2
Inland Water Aquaculture Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Anzali, Iran
AUTHOR
mina
seifzade
m_seifzadeh_ld@yahoo.com
3
AUTHOR