TY - JOUR ID - 116909 TI - Virulence of Aeromonas hydrophila in Siamese fighting fish (Betta splendens) and the bacterium susceptibility to some herbal plants JO - Iranian Journal of Fisheries Sciences JA - IJFS LA - en SN - 1562-2916 AU - Kanchan, Chutharat AU - Imjai, Puttachat AU - Kanchan, Nukoon AU - Panchai, Kwanprasert AU - Hatai, Kishio AD - Program in Aquaculture Technology, Faculty of Agriculture Technology, Rajabhat Maha Sarakham University, 44000, Thailand AD - Animal Production Technology Program, Faculty of Agro-Industrial Technology, Kalasin University, 46000, Thailand AD - Biology Program, Faculty of Science and Technologyogy, Nakhon Ratchasima Rajabhat University, 30000, Thailand AD - Borneo Marine Research Institute, University Malaysia Sabah, Malaysia, 88400 Kota Kinabalu, Sabah, Malaysia Y1 - 2019 PY - 2019 VL - 18 IS - 2 SP - 349 EP - 254 KW - Aeromonas hydrophila KW - Betta splendens KW - Antibacterial activity KW - Medicinal plants DO - 10.22092/ijfs.2018.116909 N2 - Aeromonas hydrophila was isolated from diseased Siamese fighting fish Betta splendens Regans.  The virulence of A. hydrophiala to Siamese fighting fish was conducted by intraperitoneal injection of two doses of bacterial suspensions with 7.5x107 and 7.5x105 cfu per 0.05 ml. The cumulative mortality was observed in high and low dose groups which showed 98.33 and 20 percent, respectively. Moreover, five medicinal plants extracts namely Centella asiatica, Morinda citrifolia, Melissa officinalis, Piper sarmentosum and Terminalia catappa were determined for antimicrobial activities by broth dilution method. Terminalia catappa extract showed highest antimicrobial effect of MIC and MBC (25 and 12.5 mg/ml), followed by Melissa officinalis and Piper sarmentosum with the same values of MIC and MBC (12.5 and 25 mg/ml).  Finally, Centella asiatica and Morinda citrifolia were similarly MIC and MBC (50 and 100 mg/ml).  It was concluded that five medicinal plants can use to inhibit and kill A. hydrophila in fish.     UR - https://jifro.areeo.ac.ir/article_116909.html L1 - https://jifro.areeo.ac.ir/article_116909_83533f1810440ec78ecaef79db7f0f72.pdf ER -