Agricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Shells of Bufonaria echinata as biomonitoring materials of heavy metals (Cd, Ni and Pb) pollution in the Persian Gulf: with emphasis on the annual growth sections25627111573410.22092/ijfs.2018.115734ENNima PouranguArezoo BahramiIFROH. Nasrolahzadeh SaraviCaspian Sea Ecology Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Sari, Iran.Journal Article20170312Concentrations of heavy metals (Cd, Ni and Pb) were determined in soft and hard tissues (three separated shell sections) of gastropod Bufonaria echinata as well as surficial sediments collected in October 2015 from two sampling sites located in the sub-littoral zone of Qeshm Island, Persian Gulf. There were significant differences between the sampling sites for concentrations of all the three elements in the shells and sediments. But in terms of the soft tissues, in the case of Ni and Pb significant differences between the sites could be observed. In all the cases, higher levels were observed in the samples from Suza site, which may be mainly due to the proximity of this site to the relevant anthropogenic sources. Comparison of the gained data from this study with the other relevant researches shows that in most cases the levels of the elements in the soft tissues and shells either fell within the range for other world areas or were lower. The observed increasing trends of metals accumulation in the shell sections (from older to younger sections) could be mainly attributed to the gradual increase of relevant anthropogenic pollutants in the study area, especially in Suza pier, during the recent years. Generally, it can be concluded that the shells of B.echinata could be possibly employed as a biomonitoring tool for historic metals contamination in northeastern part of the Persian Gulf.https://jifro.areeo.ac.ir/article_115734_54e477c50ccfc8fc997312594ef357d6.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Evaluation of some feeding habits of Rastrelliger kanagurta (Cuvier, 1817) in the Persian Gulf (Hormozgan Province)31933111691510.22092/ijfs.2018.118533.ENBehnam DaghooghiIslamic Azad University - Science and Research BranchFarhad KaymaramIranian Fisheries Research Institute.A VosoughiIslamic Azad University - Science and Research BranchToorag ValinassabIranian Fisheries Research Institute.Masoud MoradiIranian National Oceanography CenterJournal Article20141216The Indian mackerel (Rastrelliger kanagurta) is one of the commercial pelagic fish in the Persian Gulf and Oman Sea. In the current research, food preference index (FP), fullness index (FI), and stomach contents of Rastrelliger kanagurta were evaluated to assess the quantity and type of food which this species consumes in the study area. The samples were collected randomly from Bandar - Abbas and Qeshm Island landing sites. A total of 573 specimens were collected during 2011-2012 of which 299 specimens were female and other 274 specimens were male. The minimum and maximum total lengths were 13.9 and 35.5cm, respectively with the highest frequency in length group of 22-24cm. Results showed that 21.3% of stomachs were full, 44.5% were semi-full and 34.2% were empty. Planktons as well as random food such as bony fish were the main food of this species. Fullness index and Vacuity index for this species were calculated 21.3 and 34.2, respectively. Stomach contents analysis revealed that phytoplankton (66%) and zooplankton (34%) form the main food of Rastrelliger kanagurta. Among the phytoplankton, Bacillariophyceae (86%) was the dominant food followed by Cyanophyceae (8%) and Dynophyceae (6%). Copepods (88%) were dominant zooplanktonic foods. Encrasicholina punctifer was the only bony fish observed. Maximum and minimum of GaSI index were 2.57 (October) and 1.12 (Februrary), correspondingly. Also, condition factor and relative length gut were calculated 1.76 and 2.38, respectively. The results indicated that this fish is a relatively frugal species which consumes plankton as the main food and there is no difference in feeding of male and female.https://jifro.areeo.ac.ir/article_116915_372aff592160d0c563fc5ecc78034fbd.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Effect of salt and alkaline on the physicochemical properties of the protein isolates extracted from lanternfish (Benthosema pterotum)37138511703410.22092/ijfs.2018.118755.ENNajme OliyaeiGorgan University of Agricultural Science and Natural ResourcesMarzieh Moosavi-nasabShiraz UniversityMohammad GhorbaniGorgan University of Agricultural Science and Natural ResourcesJournal Article20150704The aim of this study was to evaluate the effect of alkaline and NaCl on functional properties of protein isolated from Lanternfish (Benthosema pterotum). In this study, extraction of fish protein was performed at different concentrations of NaCl (0.5%, 1.5%, 3% and 4.5%) at pHs 10 and 12. The results showed alkaline treatment improved the functional properties and the recovery yield of protein at pH 12 was higher than pH 10 (Phttps://jifro.areeo.ac.ir/article_117034_1770c7bdc45f3bccac418875f793a012.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Diversity and distribution of larval and juvenile fish in nearshore waters of the Southeastern Caspian Sea and Gorgan Bay33234811705010.22092/ijfs.2018.119622.ENShahin ShahlapourCaspian Sea Ecology Research CenterMohammad Ali Afraei BandpeiEcological Research Center of the Caspian SeaMahnaz RabbanihaifroNima PouranguHasan NasrolahzadehCaspian Sea Ecology Research CenterJournal Article20170618Occurrence and distribution of larval and juvenile fish was investigated in nearshore waters of the southeastern Caspian Sea including the Gorgan Bay. Samples were collected during seven surveys using a fine mesh beach-seine in five sampling sites from July 2014 to June 2015. A total of 15500 individuals mostly juvenile were captured, representing 18 species from 8 families. Most juveniles belonged to <em>Liza saliens</em>, <em>Atherina caspia</em>, <em>Neogobius pallasi</em> and <em>Neogobius melanostomus.</em> The juveniles of <em>Alburnus chalcoides</em>, <em>Vimba persa</em>, <em>Syngnathus caspius</em> and gobies <em>Neogobius caspius</em> and <em>Ponticola syrman</em> occurred in low number (relative abundance 0.1% - 9.3%). Highest number of juveniles were caught in summer season. The occurrence of larval fish in coastal waters of southern Caspian Sea was recorded for the first time (<em>Atherina caspia</em> with minimum length of 12 mm in Caspian and 9.5 mm in Gorgan Bay). In spring, early juveniles of mullet, <em>Liza aurata</em>, increased in the Caspian sites but they moved up to deeper waters by the end of the season. Based on results, the density of juvenile fish (specially <em>Liza saliens</em>) in Gorgan Bay was several times more than Caspian sites so it could be considered an important nursery ground in the region.https://jifro.areeo.ac.ir/article_117050_9a2937a0a40d608df361fe83a104252b.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Virulence of Aeromonas hydrophila in Siamese fighting fish (Betta splendens) and the bacterium susceptibility to some herbal plants34925411690910.22092/ijfs.2018.116909ENChutharat KanchanProgram in Aquaculture Technology, Faculty of Agriculture Technology, Rajabhat Maha Sarakham University, 44000, ThailandPuttachat ImjaiProgram in Aquaculture Technology, Faculty of Agriculture Technology, Rajabhat Maha Sarakham University, 44000, ThailandNukoon KanchanAnimal Production Technology Program, Faculty of Agro-Industrial Technology, Kalasin University, 46000, ThailandKwanprasert PanchaiBiology Program, Faculty of Science and Technologyogy, Nakhon Ratchasima Rajabhat University, 30000, ThailandKishio HataiBorneo Marine Research Institute, University Malaysia Sabah, Malaysia, 88400 Kota Kinabalu, Sabah, MalaysiaJournal Article20160512Aeromonas hydrophila was isolated from diseased Siamese fighting fish Betta splendens Regans. The virulence of A. hydrophiala to Siamese fighting fish was conducted by intraperitoneal injection of two doses of bacterial suspensions with 7.5x107 and 7.5x105 cfu per 0.05 ml. The cumulative mortality was observed in high and low dose groups which showed 98.33 and 20 percent, respectively. Moreover, five medicinal plants extracts namely Centella asiatica, Morinda citrifolia, Melissa officinalis, Piper sarmentosum and Terminalia catappa were determined for antimicrobial activities by broth dilution method. Terminalia catappa extract showed highest antimicrobial effect of MIC and MBC (25 and 12.5 mg/ml), followed by Melissa officinalis and Piper sarmentosum with the same values of MIC and MBC (12.5 and 25 mg/ml). Finally, Centella asiatica and Morinda citrifolia were similarly MIC and MBC (50 and 100 mg/ml). It was concluded that five medicinal plants can use to inhibit and kill A. hydrophila in fish. https://jifro.areeo.ac.ir/article_116909_83533f1810440ec78ecaef79db7f0f72.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)20521411738210.22092/ijfs.2018.117382ENİbrahim GülşafakAltuğ KüçükgülMustafa Kemal University Veterinary FacultyAzime KüçükgülJournal Article20160617To investigate the effects of carvacrol supplementation diets in rainbow trout induced by LPS, we measured gene expressions relative to pro-inflammatory and apoptosis in kidney and liver. Fish, which that were studied by four different treatments with three replicates each, was 44.71±1.33 gr. All fish were infected with E. coli LPS (25 µg ml-1) expect control and only carvacrol containing diet fed (100 µg ml-1 diet) group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 day after to determine the levels of interleukin-1β (IL-1β), interferon gamma (INF-γ), caspase 3 (Cas 3), caspase 8 (Cas 8) and by Real-Time PCR analyses. IL-1β expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (P<0.05). While IFN-γ expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and Cas 8 expressions but increased in liver. +CAR increased expressions of IL-1β and IFN-γ compared to control (up to 2-3 fold) in all tissues but not caspase gene expressions were similar in control. In all tissues, IL-1β and IFN-γ expressions increased in +CAR, expect IFN-γ in kidney. Then both genes returned to control levels. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The present results showed carvacrol had pro-inflammatory and apoptotic effects on especially liver tissue of LPS induced inflammation model of Oncorhynchus mykiss. https://jifro.areeo.ac.ir/article_117382_21b9002b7b9896d8292fa4318785238a.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Expression of estrogenic response genes to different concentration of 17ß-estradiol in male mosquitofish (Gambusia yucatana)27228211743310.22092/ijfs.2018.117433ENJaime Rendon Von OstenGisela Aguayo-DioneRicardo Dzul-CaamalMaurilio Lara-FloresEPOMEX InstituteJournal Article20160328The estrogenic effects of endocrine disrupting compounds in fish are not reversible and can reduce populations. Sensitive methods such as Q-PCR, Western blot, etc., have been used to determine changes in gene expression and this predict the effects before they become irreversible. The present study was designed to detect the expression of the estrogen receptors vitellogenin and pregnane X indicates that they are potentially useful molecular markers for detecting the presence of endocrine disrupting compounds in the environhttps://jifro.areeo.ac.ir/article_117433_3208df40c5b9937832f4cd3847525c09.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401In vitro antibacterial effect of ginger (Zingiber officinale) essential oil against fish pathogenic bacteria isolated from farmed olive flounder (Paralichthys olivaceus) in Korea38639411744610.22092/ijfs.2018.119853.ENS. HossainVeterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, KoreaB.C.J. De SilvaVeterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, KoreaS.H.M.P. WimalasenaVeterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, KoreaH.N.K.S. PathiranaVeterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, KoreaG.J. HeoVeterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, KoreaJournal Article20171106The in vitro antibacterial activity of ginger (Zingiber officinale) essential oil (GEO) was tested against five Gram-negative and Gram-positive fish pathgenic bacteria isolated from farmed olive flounder (Paralichthys olivaceus) in Korea. According to the results of disk diffusion, minimum inhibitory and minimum bactericidal concentration (MIC and MBC) tests, GEO was active against most of the bacteria. The range of MIC and MBC values of GEO against the isolates except E. tarda were documented as 0.25-4% (V/V) and 1-8% (V/V), respectively. The MBC/MIC ratio was detected as ≤4, revealing the bactericidal mode of action of GEO against most of the strains. In addition, the multiple antibiotic resistance (MAR) index values ranged from 0.09 to 0.45.https://jifro.areeo.ac.ir/article_117446_90c28fd97062e2ff869c8a2de71d206f.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Identification and investigation of species diversity and richness of the Gastropoda in intertidal zone of Bushehr Port coastal area (the Persian Gulf waters)35537011772910.22092/ijfs.2018.117729ENAbdol R. PaziraDepartment of Fisheries Sciences, College of Natural Resources, Bushehr Branch, Islamic Azad University, Bushehr, IranHadis SalehiYoung Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, IranR ObeidiYoung Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran.Journal Article20170318This study aims to identify and determine species diversity and richness of the Gastropods of intertidal zone of Bushehr seaport coastal field and assess ecological status of the area using Welch index. Sampling was carried out at 6 stations in Bushehr seaport in winter and summer seasons during 2013-2014. Sampling bed sediments was done three times in each station and Gastropod samples were collected using Quadrate (25×25 cm) , then were preserved using 4 % buffered formalin. Then preserved samples were transported to the laboratory and were identified and counted by using valid identification keys. Furthermore, physicochemical properties of water including temperature, pH, Dissolved Oxygen (DO) and salinity were also measured in each station. According to the obtained results of this study, identified samples were totally 14 species from 12 families belonging to 7 orders. The most abundant identified species in studied stations belonged to Planaxis sulctus, Cerithidea cingulate, Anachis misera species. In this study species diversity was determined by using Shannon-weiner index, dominant species were investigated using Simpson index and species richness was calculated by using Margalof index. The results indicated that the most and the least Shannon and Margalof indexes were in cold and warm seasons respectively and Shannon and Margalof indexes showed significant differences between sampled stations and seasons (P˂0.05). Moreover, the results showed that the most and the least Simpson index was in cold and warm season respectively and Simpson index showed no significant differences between sampled stations and seasons (P˃0.05). Welch index was used in order to determine the ecological status of the regions in terms of the levels of pollution. Results of the status of water quality based on the mentioned indexes in sampled stations had average and rather low/weak pollution status.https://jifro.areeo.ac.ir/article_117729_52bf5a0e5560adf6ed3196c08453fcc1.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Effect of the probiotic, Lactobacillus plantarum on growth performance and haematological indices of rainbow trout (Oncorhynchus mykiss) immunized with bivalent streptococcosis / lactococcosis vaccine28329511775710.22092/ijfs.2018.117757ENMehdi SoltaniprofessorJournal Article20170331The present study evaluated the effect of Lactobacillus plantarum as a probiotic on the growth performance and haematological parameters of juvenile rainbow trout (O. mykiss) weighing 29.6±1.8 g immunized with streptococcus/lactococcus vaccine. Fish were randomly allocated into 4 equal groups consisting of fish fed with the probiotic supplemented in diet (group P), fish immunized with streptococcosis / lactococcosis vaccine and fed with a normal diet (group V), fish immunized with streptococcosis / lactococcosis vaccine and fed with probiotic supplemented in diet (group P+V) and unimmunized fish fed (group C). The probiotic (108cells/g) was used at 1 g ∕ kg feed. Results showed that weight gain, final weight, condition factor and thermal-unit growth coefficient were improved in P+V group compared to the control one (P < 0.05).Also, feed conversion ratio in P+V group was lower than other trails (P < 0.05). In addition, white blood cell population (WBC) in groups V and P+V were significantly higher than both P and C groups (P < 0.05). Also PCV was significantly increased (P <0.05) in both P and P+V groups compared to V trail and control group. In addition, RBC and Hb levels were significantly higher (P < 0.05) in group P+V than control one. No significant difference (P > 0.05) was found in MCH, MCHC and MCV levels among the different experimental groups. Hence, application of probiotic can be considered as a beneficial dietary probiotic for improvement of growth performance and some haematological parameters in trout vaccinated against streptococcosis / lactococcosis.https://jifro.areeo.ac.ir/article_117757_3b48a0a3cee08e1d7cbf80720a8cb39c.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Quality enhancement in refrigerated tiger tooth croaker (Otolithes ruber) fillets using chitosan coating containing green tea extract22424111775810.22092/ijfs.2018.117758ENAinaz Khodanazary1 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.Amir Parviz Salati1 Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.Somayeh BohlouliAssociate professor, Department of Veterinary Medicine, Faculty of Agriculture, Kermanshah Branch, Islamic Azad University, Iran.Mitra MohammadiStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.Ehsan SadeghiAssociate professor, Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of medical Sciences, Kermanshah, Iran.Journal Article20160921The effect of chitosan coating combined with green tea extract (GTE) on the quality of tigertooth croaker (Otolithes ruber) fillets including chemical, microbiological, texture, color and sensory properties during 16 days of refrigerated storage was investigated. The changes of color different were significantly retared, and the texture parameteres and sensory scores were significantly improved in tigertooth croaker coated by chitosan, GTE and chitosan combined with GTE, compared with the control. The coincidental lowered rate of increase total volatile base (TVB) content, thiobarbitoric acid (TBA), peroxide value (POV) and free fatty acid (FFA) were obtained in tigertooth croaker coated chitosan+GTE. Chitosan in combination with chitosan had higher inhibition on microbial groeth and yielded the tigertooth croaker with higher likeness, comapared with the other treatments. Therefore, fish coated with chtoas+GTE had the lowest losses in quality during refrigerated storage.https://jifro.areeo.ac.ir/article_117758_a4035404371ea3d75059c568bae999a4.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Effects of sumithion on growth and production of phytoplankton and zooplankton in aquaculture ponds30731811775910.22092/ijfs.2018.117759ENMd. ShahjahanBangladesh Agricultural UniversityJournal Article20150922Sumithion is an organophosphorous pesticide widely used to control tiger bug in the fish larval rearing pond. The present study was aimed to investigate the effects of sumithion on plankton population abundance in aquaculture pond. The experiment was carried out with three treatments, i.e., ponds with no sumithion (T1), with 1.0 ppm sumithion (T2) and 2.0 ppm sumithion (T3). The water quality parameters, such as temperature, pH, dissolved oxygen and total alkalinity were almost unchanged throughout the study period whereas transparency, NO3-N and PO4-P values were declined with increasing sumithion concentrations but differences were not significant (P > 0.05). No distinct changes were observed in phytoplankton population densities (x cells/L). On the other hand, the zooplankton population densities (x Ind./L) were significantly (P > 0.05) decreased with toxicity of sumithion after 30 days to end of experimental period in both T2 and T3 compared to control group (T1). The ranges of pH, organic carbon (%), available phosphorus (ppm) and total nitrogen (%) of pond bottom-sediment did not differ irrespective of the treatments. This study demonstrated that sumithion has adverse effects on zooplankton which may influence the production in aquaculture pond.https://jifro.areeo.ac.ir/article_117759_3d1f3f0c0efe05a324dd28bacd18eaff.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Estimation of Otolithes ruber stock with virtual population analysis in the Northwest area of the Persian Gulf29630611780910.22092/ijfs.2018.117809ENGholamreza EskandariIranian Fisheries Research OrganizationEmad KoochaknejadAhmad HashemiJournal Article20130224Stock assessment of Otolithes ruber was carried out using Virtual Population Analysis (VPA) method in the coastal waters of Northwest Persian Gulf. Catch and length frequency data during 2002-2012 were converted to age frequency. The maximum of stock number and biomass were observed in age group 1 and 2 respectively. The minimum of stock number and biomass were observed age group 6. The maximum and minimum of catch number were in age group 2 and 6 respectively. The maximum and minimum of weight were in age group 3 and 1 respectively. The maximum and minimum of instantaneous fishing mortality were in age group 4 and 1 respectively. The maximum of fishing mortality was estimated in 2010-2011 and minimum was in 2002-2003 and 2008-2009. During 2011-2012 the stock number and biomass were estimated 29558 (*103) specimen, 7302 ton and the catch number and weight were calculated 7562 (*103), 2364 ton and fishing mortality was obtained 0.47 per year. The calculated parameters showed over-exploitation in age-groups 3 and 4. To preserve stocks and to guarantee sustainable exploitation, the prevention of increasing fishing efforts and the control of fishing activities are crucial.https://jifro.areeo.ac.ir/article_117809_78ba35fec51c39b44ef329d2a34a096a.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Effect of using various amounts of patin (Pangasianodon hypophthalmus) fish oil on physical and chemical properties of moisturizing hand cream21522311788710.22092/ijfs.2018.117887ENSamana ShabanikakroodiUniversity Putra MalaysiaAnnie ChristianusFariborz EhteshamiUniversiti Putra MalaysiaChin PingJournal Article20160917Hand cream is used for rehydrating and improving skin properties. The skin is the first point of contact with the environment. Thus having healthy skin with proper barrier properties is quite important. The beneficial effects of fish oil on skin make it a favorable ingredient to be used in hand care products. In the present study, the efficacy and stability of various hand cream formulations using 0, 1.0, 2.5 and 5.0% of fish oil were investigated and compared. The appearance, odor, texture, and pH of formulations containing 1 and 2.5% of fish oil remained stable in very good qualities during the storage, whereas the related values to the appearance and texture of formulation containing 5.0% of fish oil at the end of 6 months of storage at 45°C were significantly (<em>p</em><0.05) lower than other formulations and previous evaluations of this formulation. Weight loss was not observed in all formulations under study.https://jifro.areeo.ac.ir/article_117887_35d3d2e866afe982cbe56e5ad3ce208a.pdfAgricultural Research,Education and Extension OrganizationIranian Journal of Fisheries Sciences1562-291618220190401Blood biochemical changes in common carp (Cyprinus carpio) upon co-exposure to titanium dioxide nanoparticles and paraquat24225511817410.22092/ijfs.2019.118174ENMahdi Banaee1. Behbahan Khatam Alanbia University of Technology, Natural Resources and Environmental Faculty, Aquaculture Department, Behbahan, IranSomaye Tahery1. Behbahan Khatam Alanbia University of Technology, Natural Resources and Environmental Faculty, Aquaculture Department, Behbahan, IranBehzad Nematdoost Haghi1. Behbahan Khatam Alanbia University of Technology, Natural Resources and Environmental Faculty, Aquaculture Department, Behbahan, IranShima Shahafve1. Behbahan Khatam Alanbia University of Technology, Natural Resources and Environmental Faculty, Aquaculture Department, Behbahan, IranMaryam Vaziriyan1. Behbahan Khatam Alanbia University of Technology, Natural Resources and Environmental Faculty, Aquaculture Department, Behbahan, IranJournal Article20170410Research on eliminating organic pollutants in water by using heterogeneous photocatalysts such as nano-TiO<sub>2</sub> abound. However, the question is whether metabolites, resulting from optical dispersion of environmental pollutants, are still toxic to aquatic organisms. Therefore, this study was designed to evaluate the effect of a co-exposure to paraquat and TiO<sub>2</sub>-NPs on blood biochemical indices of common carp. Fish were exposed to 0.2 and 0.4 mg L<sup>-1</sup> paraquat with 0.125 mg L<sup>-1</sup> TiO<sub>2</sub>-NPs for 21 days under different light conditions, including natural photoperiod (16 L 8h<sup>-1 </sup>D) and complete darkness. No significant alterations in the Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP) and Creatine phosphokinase (CPK), activities and total protein, albumin, globulin, glucose, cholesterol and triglyceride levels were observed in fish exposed to TiO<sub>2</sub>-NPs and 0.2 mg L<sup>-1</sup> paraquat under normal lighting conditions. However, a significant change in blood biochemical indices in fish exposed to TiO<sub>2</sub>-NPs with 0.4 mg L<sup>-1 </sup>paraquat (16 L 8h<sup>-1</sup> D) and in fish exposed to TiO<sub>2</sub>-NPs and paraquat (under darkness). A significant decrease in the activity of Gamma-glutamyltransferase (GGT) and a significant increase in creatinine level were observed in all groups which were exposed to TiO<sub>2</sub>-NPs and paraquat. The results of this study indicate that using 0.125 mg L<sup>-1 </sup>nano-TiO2 in order to remove paraquat (0.2 mg L<sup>-1</sup>) under lighting conditions can minimize the adverse effects of paraquat and its metabolites on blood biochemical indices of fish. So, using nano-TiO2 (0.125 mg L<sup>-1</sup>) to remove paraquat under lighting conditions can significantly reduce its toxic effects.https://jifro.areeo.ac.ir/article_118174_c58ca8c63c31b7d626403aaa82562421.pdf