The effect of five isonitrogenous diets (36.6% protein), formulated by replacing 0, 25, 50, 75, and 100% of fish meal (FM) with rice protein concentrate (RPC), was investigated on the growth and tail-muscle fatty acid (FA) quality of juvenile Pacific white shrimp, Litopenaeus vannamei. The feeds were fed to shrimp (initial weight of 6.99 ± 0.08 g) five times daily to apparent satiation for 60 days. Final weight of shrimp fed with FM, 25 and 50% RPC was higher than that of shrimp fed with 75 and 100% RPC. Survival in shrimp was not significantly affected by dietary protein source and level (p>0.05). Regarding FAs, ∑n6 and PUFA increased significantly as the RPC levels increased, but the n-3/n-6 ratio, EPA+DHA, MUFA, SFA and ∑n3 were significantly declined (p < 0.05). However, tail-muscle FA composition reflected the inclusion of plant protein. Lipid quality indices (AI and TI) showed significant variation, but were very favourable for the consumers’ health. The present study suggests that RPC can replace FM up to 50% in diets for L. vannamei with no significant effect on the growth, but a decreasing trend in quality of tail-muscle FAs was observed when the RPC level increased.