Document Type: Research Paper
Department of Bioprocess Engineering, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
Citrus canker is a citrus disease species that created by the bacterium Xanthomonas citri subsp.citri. Many citrus, such as oranges, lime, and grapefruit are affected by the infectious bacteria and stems, leaves and fruits are experiencing loss. In this experiment antibacterial effects of five kinds of antibiotics including ampicillin, kanamycin, chloramphenicol, penicillin, streptomycin, Cronobacteriocin DGH2 and Enterobacteriocin DGH4 were evaluated on 107 strains of Xanthomonas citri subsp.citri. MIC and MBC data for antibiotics and bacteriocins against Xanthomonas strains were performed. According to this project, Xanthomonas strains were comparatively susceptible and resistant to Cronobacteriocin DGH2, Enterobacteriocin DGH4, ampicillin, kanamycin, chloramphenicol, penicillin and streptomycin. NIGEB-183 strain is most sensitive to these antibiotics and bacteriocins, on the other, only NIGEB-242R1 strain is resistant to chloramphenicol. Penicillin is also minimal inhibitory effects on Xanthomonas strains. Based on this case study, chloramphenicol is most antibacterial activity among of antibacterial agents and this agent is a good candidate for inhibitory activity. Cronobacteriocin DGH2 has a moderate antibacterial activity against Xanthomonas strains.