Antimicrobial activity of various extracts of Sargassum glaucescens on the antibiotic resistant organisms

Document Type: Research Paper


Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2. Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.


The antimicrobial activity of brown alga methanol, ethyl acetate, hexane, and chloroform extracts on bacteria Gram positive, Gram negative, and fungi was evaluated by using nutrient broth macrodilution test. Sargassum glaucescense was collected around the coastal area of Chabahar (south of Iran) protected marine area of the Oman sea in April and May 2015. Six pathogenic organisms including; Enterococcus faecium ATCC 51299, Streptococcus mutans ATCC 35668, Shigella boydii ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Salmonella enteritidi PTCC, 1709, Candida albicans ATCC 10231 and Aspergillus fumigatus PTCC 5009 were investigated by the broth dilution method. Methanolic Extract for six strains showed good activity amongst eight strains. Hexane extract, after methanolic extract has good effect on antimicrobial activity against five strains. All bacteria strain in this survey has showed resistance against ethyl acetate and chloroformic extracts. S. glaucescens using four various solution extract against eight different human pathogens showed an important antimicrobial and antifungal activity. However, more investigation has to be done on separation, purification and detection of the active ingredients in order to recognize their antifungal and antifungal activity.