Document Type: Research Paper
quatic Animal Health &amp; Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Aquatic Animal Health &amp; Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Aquatic Animal Health and management Department,Iran Veterinary organisation, Tehran, Iran
During a fish disease outbreaks in 2015-2016 suspected to viral hemorrhagic septicemia virus (VHSV), moribund rainbow trout fries were obtained from fish farms. Samples of kidney, heart and spleen tissues were taken [X1] for examination. The full coding region of the G gene was amplified by RT-PCR reaction. Genotyping was carried out by phylogenetic analysis with reference sequences of four VHS genogroup. A phylogenetic tree was constructed with the maximum likelihood method and the Kumara 2- parameter substitution model by using MEGA software version 6.06. A maximum likelihood phylogenetic analysis reveals that all the Iranian VHS strains belonged to clade Ia-2 of genotype Ia. The intra- sequence comparison of Iranian VHS strains ranged from 99.1% to 100% (average was 99.55%). In this study, two strains (Chaharmahal and Bakhtiari (1) and Chaharmahal and Bakhtiari (3) showed 100% identity but 0.1% differences with Isfahan strain. The Iranian strains belonging to European genogroup are related (99% identity) to VHSV from recent Italian strains (VHSV/O.mykiss/I/TN/133/Apr10).
[X1]نیازی به ماضی نقلی نیست ، مجهول گذشته ساده صحیح است.