Effect of enviromental temperature on heat shock proteins (HSP30, HSP70, HSP90) and IGF-I mRNA expression in Sparus aurata

Document Type: Research Paper

Authors

University

Abstract

Ambient temperature is one of the most important environmental factors affecting physiological mechanisms and biochemical reactions of living things. Thus the effect of ambient temperature on HSPs and IGF-I gene expression levels in liver and muscle tissue of Sparus aurata were investigated in this research.

The levels of HSPs, and IGF-I gene expression of liver and muscle of Sparus aurata were analyzed in by qRT-PCR. The experiment was done   in July (27 ◦C) and  January (18◦C). HSP70 mRNA relative expression levels in the muscle on January were significantly  higher than July (approximately 1.7 fold), whereas HSP30 gene expression in liver on July was increased by 2.0 fold (P<0.05). Transcription of other heat shock proteins and IGF-I were not affected by the change in water temperature. The HSP findings of the research show that these proteins are important and sensitive in the average adaptation

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