Characterization of HSP70 and HSP90 genes of tropical abalone (Haliotis diversicolor squamata) and their expression under salinity induced stress

Authors

1 Research Center for Fishery, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 47 Cibinong, Bogor Jawa Barat 16912, Indonesia.

2 Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46 Cibinong, Bogor Jawa Barat 16911, Indonesia.

3 Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, Jl. Flora, Bulaksumur, Yogyakarta, 55281, Indonesia.

10.22092/ijfs.2023.129864

Abstract

Salinity is an environmental factor that affects abalone, H. diversicolor squamata stress and survival in the hatchery and grow-out area. To understand the protective mechanism of HSP70 and HSP90 under salinity stress, a completely randomized design, and one way ANOVA test were carried out with 95% confidence interval. To characterize heat shock protein genes, we used target clones and target plus clones to obtain partial length sequences of two heat stress response-related genes: (1) heat shock protein 70 (HSP70) and (2) heat shock protein 90 (HSP90). The HSP70 and HSP90 genes contain 201 bp and 302 bp which encode 38 and 87 amino acids, respectively. The results of multiple sequence alignment showed that HSP70 and HSP90 sequences were highly conserved compared to other species. Real-time polymerase chain reaction (PCR) results showed that HSP70 and HSP90 were salinity dependent and HSP70 and HSP90 gene expression was quantified by Quantitative Real-Time PCR of hemolymph and leg muscles showing 10ppt salinity shock for 12 h showing higher HSP70 and HSP90 mRNA expression levels higher than the control group at 32 ppt and decreased expression thereafter. Experimental results suggest that these two genes may play an important role in responding to environmental stress caused by decreased salinity. Thus, this study established a theoretical foundation for further in-depth study of mechanisms of protection of abalone molecules against salinity stress.

Keywords

Iranian Journal of Fisheries Sciences
Volume 22, Issue 4
July 2023
Pages 809-827

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